During a visit to Lichfield Cathedral on Monday, King Charles was heckled by a protester who asked him about Prince Andrew's connections with convicted sex offender Jeffrey Epstein.
The man inquired how long the monarch had been aware about his sibling's association with Jeffrey Epstein, and also questioned whether he had instructed the authorities to "cover up" for Andrew.
Despite the protest, there were crowds of supporters and applause for the King, who was visiting the region for an event.
Prince Andrew's associations with Epstein have been under renewed focus in recent weeks. The royal, who gave up his titles earlier this month, has categorically denied any misconduct.
"How long have you been aware about Andrew and Epstein? Have you asked the authorities to conceal for Andrew?"
The King ignored the questions but continued greeting fans, some of whom shouted "Long live the King". One supporter can be overheard asking him for a embrace.
A female bystander outside the cathedral was also observed telling the heckler to "leave" and to "be quiet".
The controversy over the Duke of York's connections to Epstein has been revived by sexual abuse claims repeated in Virginia Giuffre's posthumously-published memoir.
In the memoir, the accuser claims she had sex with the prince on three separate occasions, including one instance with Jeffrey Epstein and "several other minors"
Prince Andrew, who agreed to a out-of-court agreement with the plaintiff in 2022, has repeatedly denied any misconduct. In 2019, he told BBC Newsnight he did not remember meeting Ms Giuffre "at all", and that they "did not have any sort of sexual contact."
But recently, new inquiries have been asked about how he is able to finance his way of life despite not being a working royal.
The revelation that he has only ever paid a nominal fee on his Royal Lodge has intensified the examination over his living arrangements - even though he made large payments up front, including for renovations.
The political party said he should be called in before MPs to give testimony about his lease of his lodge.
But last Thursday, the authorities refused to give MPs time to discuss the prince's status or his home, despite increasing calls for scrutiny.
The royal household has not commented on whether the Duke of York might relocate of his current residence - or where he might go.
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Ashley Rasmussen
Ashley Rasmussen
Ashley Rasmussen